Relationship between gene expressions and related terpenes at three developmental stages in Artemisia annua - (IJAAR)

Abstract

Importance of terpenes in different aspects of human’s life, makes it necessary to determine the relationship between them and their genes just in order to fathom such relationship for further exploitation of their production process, either increasing the volume of synthesis or changing the mixture of terpene content, for exponentially demanding for these invaluable compounds. To do so, In this study some genes in 2-c-methyl-d-erythritol 4- phosphate and Mevalonate pathways namely Farnesyl diphosphate synthase, Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase and Isiopentenyl diphosphate isomeras were evaluated in three of Artemisia annua tissues including leaf, bloom and flower. Real-time PCR results of those genes compared with the information taken from GC-Mass outputs of terpenes, yields the final result of this research. Results showed, relationship between genes and terpenes was not similar in tissues. In certain tissues, these genes play a limiting role, while in others, some other downstream genes were possibly more important, still the effect of post-transcriptional modification cannot be ignored. In the end, although those investigated genes in this research are somehow crucial in terpene production, in order to make a more obvious picture of terpene synthesis, evaluation of more immediate genes to terpenes, particularly terpene synthases, and proteomics data of these genes needed to make the relationship between RNA transcrips and metabolites more reliable.